Handbook of Experimental Ultraviolet Absorption Spectroscopy

 ‘Handbook of Experimental Ultraviolet Absorption Spectroscopy’ could be an integral part of library as it explains methods for instrument performance, solvents effects, estimation for single and multicomponent formulation and various ways of validation. In instrument performance, procedure for calibration of absorbance scale of ultraviolet-visible spectrophotometer is described. Secondly, effect of organic, alkaline, acidic and hydrotropic solvent on wavelength maxima, bathochromic, hypsochromic shift has been coated.  This book also covers single point, double point standardization, calibration curve methods for estimation of drug for single component as well as simultaneous equation, absorbance ratio, derivative, dual wavelength and area under curve methods for estimation of two components. Three wavelength methods are also described for formulations containing three components. As developed analytical methods must be validated to provide reliable data for regulatory submissions.  The results from method validation can be used to judge the quality, reliability and consistency of analytical results. For this purpose, book describes how to studyBeer’s limit, absorptivity, specific absorbance and molar absorptivity constant, precision, accuracy, recovery study, calculation of LOD and LOQ as per the ICH guideline. The book script is focused at UG and PG level for pharmacy students as well as for M.Sc. students which provides basic platform for pharmaceutical analysis and research as well