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Whoever wishes to adopt microsurgery as a technique within their therapeutic repertoire should know that it demands skills that develop in the microsurgery laboratory. This handbook intends to aid in the acquisition and development of those skills necessary to perform a microsurgical procedure of excellence. Keeping up with the changes observed in educational processes, this guide uses explanatory videos in all the techniques described making the reading of the handbook more dynamic and provides unequaled and detailed descriptions of each technique, through the tips the author offers in the different gestures. The techniques introduced increase in complexity, ranging from artificial models to free flap making in live models. The Handbook begins with simple exercises and leads you through complex practices so that the reader gains the necessary skills and abilities progressively. This handbook is intended for human and animal health professionals of different areas. Therefore, it can be used by veterinarians, dentists and surgeons of different specialties. This paper aims at coping with the lack of teachers in the microsurgery laboratories, which hinders good training, and can be used as reference material for various activities throughout the microsurgical learning process at university or for individual learning.